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KMID : 1094720160210030439
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Biotechnology and Bioprocess Engineering
2016 Volume.21 No. 3 p.439 ~ p.445
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Heparan sulfate proteoglycan synthesis in CHO DG44 and HEK293 cells
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Lee So-Jeong
Kim Mi-Gyeom
Kim Nu-Ry
Heo Won-Do
Lee Gyun-Min
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Abstract
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Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells are the most popular host cells for transient gene expression (TGE) of therapeutic proteins. These host cells require high transfection efficiency in order to enhance TGE. Heparan sulfate proteoglycan (HSPG) at the cell surface is known to regulate endocytosis for gene delivery. The HSPG expression in CHO DG44 and HEK293E cells was investigated in an effort to enhance the TGE. Immunostaining of HSPGs followed by confocal microscopy and flow cytometry analyses revealed that CHO DG44 cells possessed a higher amount of cell-surface and intracellular HSPGs than HEK293E cells. The mRNA levels of the representative enzymes involved in the HSPG biosynthesis in CHO DG44, which were determined by quantitative real time PCR, were quite different from those in HEK293E cells. Taken together, the results obtained here would be useful in improving TGE in CHO DG44 and HEK293E cells through genetic engineering of HSPG synthesis.
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KEYWORD
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CHO cells, HEK293, Heparan sulfate proteoglycan (HSPG), HSPG biosynthesis
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